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KMID : 1098420060140060354
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Korean Journal of Medicinal Crop Science
2006 Volume.14 No. 6 p.354 ~ p.359
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Cytogenetic Analysis Using Mitosis, Meiosis Chromosomes and bicolor Fluorescence in situ Hybridization of Bupleurum latissimum Nakai
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Kim Soo-Young
Bang Jae-Wook
Lee Joong-Ku
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Abstract
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Chromosome analysis using mitosis, meiosis and bicolor FISH were carried out in Bupleurum latissimum Nakai, which is one of the endemic plants in Ulleung island of korea. The somatic methaphase chromosomes number of this plant was 2n = 2x = 16 and the chromosome complements consisted of six pairs of metacentrics and two pairs of submetacentrics. The size of chromosomes ranged 2.40{sim}4.20 {mu}m and NOR (nucleolus organizer region) chromosome did not observed using conventional staining. In meiosis chromosomes, metaphase-I and anaphase-I were observed. Metaphase-I anaphase-I showed 8 bivalents and chromosomes migration to make two daughter cells. Using bicolor FISH, one pair of 5S and 45S rDNA signals were detected on the centromeric region of chromosome 3 and the end of short of chromosome 2,respectively. We also observed the NOR using 45S rDNA probe.
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KEYWORD
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Bupleurum latissimum Nakai, mitosis, meiosis, karyotype, rDNAs, bicolor FISH
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